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Ratiometric image analysis was conducted using a Python script (available from The background was subtracted for each channel and the ROIs (=transfected cells) were determined by thresholding or with the Cellpose 40 algorithm in the channel of the FP Lens Mount:Nikon Z https://i.ytimg.com/vi/JjlaJs4vl6Y/sddefault.jpg HPLC Method for Analysis of Glutathione oxidized (GSSG) on Primesep 100 Column | SIELC Technologies B Vitamins
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