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100 μL of HCT116 cells (WT, ∆ ADH5 , ∆ ADH5 complemented, ∆ GCLM , or ∆ ADH5 ∆ GCLM ) were seeded at a concentration of 2 × 10 3 or 4 × 10 3 cells/well
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On the following day, cell culture medium was replaced with fresh medium containing 100 μM GSH, or 100 μM N-acetylcysteine (NAC) or 10, 50, and 100 μM LCC
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