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For cell sorting of Lenti-X 293T, cells were rinsed twice with 500 μL PBS and then treated with 300 μL Non-enzymatic Cell Dissociation Solution (13151014, Gibco) for 4–6 min at 37°C to detach cells

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Thus, we performed phasor fluorescence lifetime imaging (phasor-FLIM) to measure fluorescence lifetime ( τ f ), which can be correlated to the molecular self-assembly 21,34

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