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Transferred intratumoral OT-1 GPX4 cKO CD8 + T cells displayed a similar exhausted-like phenotype (expressing PD-1 and Lag-3) (Supplemental Figure 1M) but an increased stem-like phenotype (expressing Ly108 and CD69) (Supplemental Figure 1N) compared with control CD8 + T cells
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This was followed by three pairs of monopolar needles stabbed 3 mm deep into the tail at measurements of 10, 60, and 100 mm caudal to the base of the tail
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