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Generation of CRISPR/Cas9 knockout colonies Drp1 CRISPR/Cas9 KO cell lines were generated from HT22 cells by the transient transfection of a plasmid (pSpCas9(BB)-2A-GFP, Addgene, MA, USA) containing the single guide RNA with the sequence addressing the GTPase Domain of the Drp1 protein (GGTCATGGAGGCGCTGATCC), a GFP reporter and the Cas9 nuclease, following fluorescence-activated single-cell sorting into 96-well-plates in the MoFlo Astrios Cell Sorter (Beckman Coulter GmbH, Krefeld, Germany)

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Growth hormone and hepatic lipoprotein metabolism