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Cells were spun at 1,000 rpm for 5 min at 4 °C, dissociated for 5 min with 100 µl ice-cold lysis buffer, neutralized with 1 ml wash buffer, spun at 1,000 rpm for 5 min at 4 °C and resuspended in chilled nuclei buffer included in the Single Cell Multiome ATAC kit (10x Genomics)

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[DOI] [Google Scholar] 85.Wu X., Li J., Hu C., Zheng Y., Zhang Y., Li J., Li M., Xiao D., Lu L., Huang Y., et al