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125 Bacterial or viral infection is a common trigger for COPD exacerbations, and exposure to LPS induces p38MAPK activation in rat peritoneal macrophages and dendritic cells as well as an increase expression of inflammatory mediators
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The PCR reaction was carried out in a master cycler (Applied Biosystem) with following reaction cycle: 3 min of initial denaturation (94 °C) followed by 35 cycles of denaturation for 1 min (94 °C), respective annealing temperature for 1 min, extension for 1 min (72 °C) and a final extension step for 7 min (72 °C)
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