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Cell Cycle Assay When the confluence reached ~70–80%, cells (1 × 10 6 /ml) were collected and fixed in ethanol (70%) at a temperature of −20°C for more than 18 h
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Gene expression analysis revealed that SH modulates the expression of genes involved in β-1,3-glucan synthesis and transport, including upregulation of ZAP1 , ADH5 , XOG1 , and FKS1 , suggesting its potential mechanism involves enhancing cell wall-targeted antifungal responses (Shao et al., 2017
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